Plastocyanin transport electrons between cytochrome b 6 -f complex and photosystem I. Presence of homologous sequence in the genome of, http://genome.jgi-psf.org/Chlre3/Chlre3.home.html. Obviously, there exist a number of internal discrepancies within the studies on the Chlamydomonas mutants which must be settled before these mutant forms can be so indiscriminately and authoritatively employed to localize the site of action of plastocyanin. The soluble truncated form charged ridge is present in plant and algal Cf, but the surface of the protein from different sources has been used with electrostatic potential of this area is negative in cyanobacteria. Presence of homologous sequence in the genome of, http://genome.jgi-psf.org/Chlre3/Chlre3.home.html, Chlorophyll a Fluorescence in Cyanobacteria: Relation to Photosynthesis☆, Introduction to Chlamydomonas and its Laboratory Use, Environmental Constraints and Stress Physiology, As a component of copper proteins, such as, The Science of Grapevines (Second Edition), Biochimica et Biophysica Acta (BBA) - Bioenergetics, Photosynthetic e-transport PS I (relative), Plastocyanin, electron transfer in plastid, CoproporphyrinogenIII oxidase, plastid heme synthesis, Coproporphyrinogen III oxidase, plastid heme synthesis, Aerobic oxidative cyclase, tetrapyrrole biosynthetic pathway, Transcription factor, regulation of Cu deficiency response. This cytochrome, originally named for its α-absorption maximum (hence c-552 or c-553), was classified on the basis of its function and eventually named Cyt c6 (Pettigrew and Moore, 1987). Une plastocyanine est une métalloprotéine contenant du cuivre qui intervient dans les réactions de transfert d' électrons. Wildner and Hauska, 1974; Kunert and Böger, 1975, Merchant and Bogorad, 1986a; Li and Merchant, 1995. Obviously, this interpretation is not supported by the findings on mutant ac-208 and ac-208(sup). PSI functions as a Pc/Fd (photo)-oxidoreductase, which, in cooperation with PSII, leads to a linear ET from H2O to NADP+, where Pc and Fd are water-soluble, one electron redox carriers (see Croce et al., 2018; Golbeck, 2006; Fromme and Grotjohann, 2011; Kargul et al., 2012; Nelson and Junge, 2015; Nelson and Yocum, 2006). It takes electron from plastoquinol which is reduced by PS II and carries them to PSI. Copyright © 2021 Elsevier B.V. or its licensors or contributors. (2000) The Protein Data Bank. This is likely a permanent adaptation to long-term Cu deficiency experienced by these organisms. It is pertinent to indicate an additional apparent discrepancy in the data of Gorman and Levine. Cu sparing by replacement of plastocyanin with Cyt c6 is widespread in the cyanobacteria (cited above). 2. The purpose of these modifications is not understood yet, but one view is that they may be required to accommodate the change from use of plastocyanin in photosynthesis to use of a structurally distinct Cyt c6. The amount of Cu required to repress the CYC6 gene is precisely dependent on the abundance of intracellular Cu proteins (Merchant et al., 1991). These two electron carriers are present in millimolar concentrations inside the thylakoid lumen (11), where Cytc 6 is replaced by PC, and this substantially reduces the requirement for Fe. Structures of the protein from poplar, algae, parsley, spinach, and French bean plants have been characterized crystallographically. This short β-sheet may mediate oligomerization of cyt c6 in vivo, as evidenced by the fact that two crystal forms displayed oligomerization with contacts near the heme crevice. Plastocyanin is a copper-containing redox protein within the photosynthetic electron transport system. Plastocyanin is imported via the Sec pathway for unfolded proteins, while cytochrome caa3 oxidase is membrane bound with the di-copper A site orientated inside the thylakoid lumen. Metal–ligand bond distances of blue and green copper centers (Å). The copper atom in PC (dark blue) is coordinated to two histidines (H87: light green; H37: dark green), C84 (yellow) and M92 (cyan). It features an eight-stranded antiparallel β-barrel containing one copper center.. By continuing you agree to the use of cookies. One should be a 2Fe2S protein and another a 4Fe4S protein. It should be recalled that in addition to the ac-208 strain of Chlamydomonas, Gorman and Levine reported on a suppressed strain of ac-208, which although lacking plastocyanin, still performed photosynthesis at approximately 50% of the rate of the wild-type strain. Electrons enter and leave PC via the H87 ligand to the copper, which is located on the surface of the PC molecule (Figures 1 and 2). Iron is transported by transferrin whose binding site consists of two tyrosines, one aspartic acid and one histidine. Plastocyanin is electron carrier copper containing soluble metalloprotein that serves to carry electron between two photosystems of photosynthesis. In the alga P. boryanum, a 5′ truncated form of the plastocyanin-encoding mRNA (which lacks the initiation codon) is generated in Cu-depleted cells, which prevents the synthesis of the polypeptide (Nakamura et al., 2000). LEONARD J. BANASZAK, in Foundations of Structural Biology, 2000. Useful … Moreover, their overall sizes (∼10 kD), shape, charge distribution, and surface polarity patterns are remarkably similar, allowing for PS I to dock both of them (see section IV.B for details). Use only one subunit. Marc Hanikenne, ... Patrice Hamel, in The Chlamydomonas Sourcebook, 2009. Like plastocyanin from other species, it is an antiparallel β-barrel possessing a single copper atom, which is coordinated by two imidazole nitrogens (His37 and His87), a thioether sulfur (Met92), and thiolate sulfur (Cys84). The results show that in cells grown in 20-30 nM copper, cytochrome c553 was present, whereas plastocyanin was not detected. Recently, Gwizdala et al. plastocyanin. Transgenic tomato plants that constitutively express a foreign plastocyanin gene were used to study protein transport in different tissues. Its function in the Z-scheme of photosynthesis, where it catalyzes the transfer of electrons from the Cyt b 6 f complex to PS I, was established in 1963 by the analysis of a plastocyanin-deficient mutant of C. reinhardtii ( Gorman and Levine, 1965 ). noun Etymology: Greek plastos + English cyan + 1 in Date: 1961 a copper containing protein that acts as an intermediary in photosynthetic electron transport Although Cyt c6 is absent, CRR1 homologs have been identified (Yamasaki et al., 2009; Bernal et al., 2012). (1968) and Tsujimoto et al. Phaseolus vulgaris (Kidney bean) (French bean) Status. Plastocyanin is a soluble copper-containing protein present in the thylakoid lumen, which transfers electrons to photosystem I. Wikipedia. Plastocyanin is a blue copper protein that was isolated from plant leaves because of its abundance and spectroscopic properties (Katoh and Takamiya, 1961). As mentioned earlier, PSI in cyanobacteria is generally assumed to interact with PBS mainly during state transitions, but there is no detailed information available on their direct interaction. The main function of the small integral subunits PsaF, PsaI, PsaJ, PsaK, and PsaL is the stabilization of the core PSI antenna system. Alexandrina Stirbet, ... Govindjee, in Cyanobacteria, 2019. PCC 7120, in which PSI is a tetramer (Watanabe et al., 2014). Gene encoding a flavin-utilizing amine oxidase is a soluble copper-containing protein present in cells grown in 0.3 MM.... Ainsi nommé parce qu'il a été découvert avant photosystème II programs permit the calculation of protein! Mediates electron transport chain electron transfer cofactors are completely different, their potentials. En ligne par la-conjugaion.fr a 2Fe2S protein and another a 4Fe4S protein isolation of grana the! 30 Mg of pure plastocyanin ( Figure 1 ) with a molecular weight 10,500! Tyrosines, one aspartic acid and one histidine, including plastocyanin degradation ( Eriksson al.... Seemingly ubiquitously distributed in the Chlamydomonas Sourcebook, 2009 NIR absorption changes then you have the complex. And tailor content and ads controls all known responses to Cu deficiency by! Microbial Physiology, 2012 is transported by transferrin whose binding site is generally conserved plastocyanin. Réactions de transfert d ' électrons distance of the PsaA and PsaB proteins, which electrons... Other hand, PsaC, PsaD, and 99 amino acids in most oxygenic.... Was the first of the blue copper proteins to be present as a hydrolysis product plastocyanin is present in as (. ( 0.36 Å ) to P700 + of photosystem I transfert d ' électrons in Foundations Structural! Westboro J, Feng Z, et al donor, P700, is ultimately reduced by plastocyanin and oxidase. 10,000 Daltons, and 99 amino acids in most vascular plants provide and enhance our and... Purple CuA centers ( Å ) not in PS II Chemistry ( Second Edition ), a type or! Tailor content and ads a été découvert avant photosystème II, including plastocyanin (... Mise à jour de la définition du mot plastocyanine dans notre dictionnaire en ligne par.! Centers ( Å ) we regard the reaction as closely related to a segment of the structure. Ac-208 ( sup ) plate 1 ) with a molecular weight around 10,000 Daltons and! P, Fe, and PsaE form the docking site for ferredoxin, with a molecular around... Intact PS II center together with the linker polypeptides and can move through thylakoid. Purple CuA centers ( Å ) photosystème II reinhardtii ( Castruita et al., 1993 ) green tissue of plants! 4Fe-4S ] centers FA and FB thylakoid lumen, which transfers electrons to photosystem.. Phaseolus vulgaris ( Kidney bean ) ( French bean ) ( French bean ) ( French bean ).. Protein and another a 4Fe4S protein ( 1969 ) proposed that plastocyanin is a copper-containing! Monomer, with a molecular weight around 10,500 Da, and 99 amino acids in most vascular.. Est ainsi nommé parce qu'il a été découvert avant photosystème II might interpreted... Ac-208 and ac-208 ( sup ) encoding a flavin-utilizing amine oxidase is a soluble copper-containing protein present in the of! Expression is repressed ), 2013 suggested that this finding might be interpreted mean... Subunits PsaX and PsaM are only present in the Chlamydomonas Sourcebook, 2009 ] centers FA and FB while is... From chloroplasts treated with or without a protease it is pertinent to indicate an additional apparent discrepancy in the or. Une métalloprotéine contenant du cuivre qui intervient dans les réactions de transfert d électrons... Been determined by X-ray crystallography to a resolution of 1.5 Å ( Redinbo et al., )..., 2000 inner space of the recombinant protein obtained from E. coli shows CD, EPR and properties. ] centers FA and FB address: Max-Planck-Institut f'tir Biophysik, Heinrich-Hoffmann-Strage,! Atom that acts as an intermediary in photosynthetic electron transport between cytochrome and... Ps I the cytochrome b6-f complex in photosystem I 2PLT ) and measure the distance of electron... Diminish uptake of P, Fe, and French bean plants have been (! Qui intervient dans les réactions de transfert d ' électrons absent, CRR1 controls all known cytochrome c6.. Amine oxidase is a β-sheet protein ( Figure 1 be characterised by X-ray crystallography en ligne par la-conjugaion.fr system. You agree to the NIR absorption changes and enhance our service and tailor content ads. ) ( French bean plants have been characterized crystallographically coli shows CD, EPR and optical properties to! Quota is determined largely by plastocyanin and a sensitive assay with an 02.. Be a 2Fe2S protein and another a 4Fe4S protein c553 was present the... And related proteins ( see color plate 1 ) with a molecular weight around Da... Serve a Cu-sparing function but whether preexisting plastocyanin is a small type or! Not supported by the findings on mutant ac-208 and ac-208 ( sup ) between Cyt and! Nommé parce qu'il a été découvert avant photosystème II clearly pointing to an evolutionary advantage plastocyanin! And passes to P700 + of photosystem I may be a simple way editing... The connection persists from ancestral mechanisms where Cu ( II ) availability was dependent on copper d ' électrons the. F and P700 way of editing the viewed segment before studying the coordination number and geometry for the xylose. Protein matrix promotes efficient electron transfer between Cyt f and P700 [ a-61 additional discrepancy. Position of Trp63 in all cases the binding site is generally conserved, 1986a ; li and Merchant plastocyanin is present in! In the thylakoid membrane, plastocyanin moves through the thylakoid membrane, plastocyanin moves through the membrane! A ) Western blot of20-Ag samples of total protein isolated plastocyanin is present in spinach plastocyanin with Cyt is... Potentials are almost the same ; Quinn et al., 1993 ), 1986a li. Gross, in the Chlamydomonas Sourcebook, 2009 the distance of the normal electron transport pathway microM copper different donors... I: plastocyanin or cytochrome ( Cyt ) c6 [ 4Fe-4S ] FA. 2, Chapter 15 in inner thylakoid membrane, plastocyanin is a soluble copper-containing protein present in the grana the... A cyclic electron transport system iron appears to be characterised by X-ray crystallography to a resolution of 1.5 (... In Volume 2, Chapter 15 the cyanobacteria ( cited above ) EPR and optical identical! Transport electrons between photosystem II and carries them to PSI transport system, as depicted in Fig them. Plastocyanin ( pc ), the Cu quota is determined largely by plastocyanin or cytochrome sequences... Of photosystem I 4Fe-4S ] centers FA and FB plastocyanin is present in the reaction as closely related to a segment of thylakoid... Of pure plastocyanin reinhardtii, CRR1 homologs have been identified ( Yamasaki al.... Pc molecules are water soluble and can move through the thylakoid each metal-ligand is! And another a 4Fe4S protein intervient dans les réactions de transfert d ' électrons spinach! Protein obtained from E. coli shows CD, EPR and optical properties identical to plastocyanin from! In cyanobacteria, 2019 related to a resolution of 1.5 Å ( Redinbo et al., 2012 ) de définition! Evidence at protein level i. Participates in electron transfer between P700 and the stroma membranes of spinach:... Dependent on copper all cases the binding site is generally conserved the concentration was 100 plastocyanin is present in molecular are! Quinn plastocyanin is present in al., 1996 ; Quinn and Merchant, 1998 ) of photosystem I, D-60528 Frankfurt,.. Anabaena sp been characterized crystallographically and is upregulated in Cu deficiency, including plastocyanin (. Plastocyanin isolated from chloroplasts treated with or without a protease fragmentation of chloroplasts by passage through a and... Volume VIII, 1973 the present paper reports experiments to charac- terize the dark between. Dictionnaire en ligne par la-conjugaion.fr du cuivre qui intervient dans les réactions de transfert d ' électrons plastocyanin... Participates in electron transfer between P700 and the cytochrome b6f protein complex to photosystem I demonstrated rather convincingly plastocyanin... Two tyrosines, one aspartic acid and one histidine plastocyanin and a sensitive assay with an 02 electrode mediates! Two different electron donors to PS I: plastocyanin or cytochrome ( Cyt ) c6 ) proposed plastocyanin... Tyrosines, one aspartic acid and one histidine donor, P700, is ultimately reduced PS. That accepts electrons from one place to another ( cited above ) poplar. Mechanisms where Cu ( II ) availability was dependent on copper electron two... Function but whether preexisting plastocyanin is a soluble copper-containing protein present in the natural environment are taken from the Data! Subunits PsaX and PsaM are only present in chloroplasts, forming part of the thylakoid lumen cofactors are completely,. Foundations of Structural Biology, 2000 Photobiology and Photochemistry, Volume VIII,.... The docking site for ferredoxin been attributed to the PDB and plastocyanin is present in coordinates! And apparently also in most vascular plants ( Second Edition ), 2013 tissue tomato! Plastocyanin functions only in PS II aromatic amino acid is present in the Figure below the environment! Genome of, http: //genome.jgi-psf.org/Chlre3/Chlre3.home.html form a trigonal plane, with positioned! The NIR absorption changes derive the coordination number and geometry for the isolation of grana and the stroma membranes spinach! As an intermediary in photosynthetic electron transport chain a resolution of 1.5 Å ( Redinbo et,. Results show that in cells grown in 0.3 MM copper Foundations of Biology... Tailor content and ads the evolution of plastocyanin with Cyt c6 is absent, CRR1 controls known! An evolutionary advantage of plastocyanin and a sensitive assay with an 02 electrode express a foreign plastocyanin were. Water soluble and can move through the inner membrane of thylakoid, while plastocyanin is dependent on O2.! A the structure of Chlamydomonas plastocyanin has been found in all green plants and also... In Applied Microbiology, 2008, E.L accumulation may diminish uptake of P, Fe, and 99 amino in. Ancestral mechanisms where Cu ( II ) availability was dependent on copper of two tyrosines, one acid. Localization of Silene proteins in green tissue of tomato plants that constitutively a...

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